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Anti-Smooth Muscle Actin - Mouse (B4)
Price:
$ 250.00
 
B4 reacts selectively with enteric smooth muscle gamma-actin at high dilutions and does not react to either cytoplasmic ß- or gamma-actin.
Details:

   Download datasheet

Catalog Number LMAB-B4
Quantity 100 µl
Price $250.00
Category Monoclonal
Specificity Reacts selectively with enteric smooth muscle
Immunogen Chicken gizzard actin
Isotype IgG1/Kappa
Applications &
suggested dilutions
To be selective for smooth muscle actin:
Immunoblots - 1:2000
Indirect immunofluorescence - 1:100-1:500
Immunohistochemistry - 1:500
Blocking conditions: human specimens: 2% serum, mouse specimens: MOM kit (Vector Labs)
Supplied As Acites fluid containing 0.01% Na azide.
Storage Storage at -70oC recommended, however the antibody is stable for months at -20oC and for weeks at 4oC. Multiple freeze/thaw cycles not recommended.


References:

Lessard, JL.  'Two monoclonal antibodies to actin: one muscle selective and one generally reactive.'  Cell Motil Cytoskeleton. 1988;10(3):349-62.

Sawtell NM, Lessard JL.  'Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.'  J Cell Biol. 1989 Dec;109(6 Pt 1):2929-37.

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