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Dong M et al.
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Mol. Biol. Cell 2008;19:2620-2630.

Stahlman MT et al.
Expression of ABCA3 in developing lung and other tissues. J Histochem Cytochem 2007;55:71-83.

Ad5-CMV-HDAC4
Price:
$450.00
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HDAC4 (flag)
Details:

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Catalog Number JMAd-01
Quantity 0.5 ml
Price $450.00
Category HDACs
Gene HDAC4 (flag)
Vector Construction

Mouse cDNA for HDAC4 (Histone deacetylase) cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cDNA for HDAC4 expression cassette with a  Flag epitope, generating a replication deficient recombinant virus. 

Supplied As Crude HEK293 cell lysate
Storage

Store at -80°C. Multiple freeze/thaw cycles not recommended.

Epitope Tag flag
QC Testing Western Blot
Titer

Approx 1E8/ml


References:

Dai YS, Xu J, Molkentin JD. The DnaJ-related factor Mrj interacts with nuclear factor of activated T cells c3 and mediates transcriptional repression through class II histone deacetylase recruitment. Mol  Cell Biol. 2005 Nov;25(22):9936-48.

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et. Al., J. Biol. Chem (1992) 267:25129-25134.

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