Mouse cDNA for HDAC4 (Histone deacetylase) that is mutated at S246A, S467A, and S632A.  It was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cDNA for HDAC4-3SA expression cassette with a Flag epitope, generating a replication deficient recombinant virus.