| Catalog Number |
JMAd-10 |
| Quantity |
0.5 ml |
| Price |
$450.00 |
| Category |
Calcineurin/NFAT |
| Gene |
NFAT-luciferase reporter (reporter adenovirus for nuclear factor of activated T-cells activity and calcineurin activation) |
| Vector Construction |
NFAT- luciferase reporter that contains 9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase (lacking the CMV promoter) was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.
|
| Supplied As |
Crude HEK293 cell lysate. |
| Storage |
Store at -80oC. Multiple freeze/thaw cycles not recommended.
|
| Epitope Tag |
None |
| QC Testing |
None |
| Titer |
Approx 1E8/ml |
References:
Wilkins BJ, Dai Y-S, Bueno OF, Parsons SA, Xu j, Plank DM, Jones F, Kimball TR, Molkentin JD. Calcineurin/NFAT coupling participates in pathologic, but not physiologic cardiac hypertrophy. Circ. Res. 94:110-118 2004.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992) 267:25129-25134.
