NFAT- luciferase reporter that contains 9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase (lacking the CMV promoter) was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.

Store at -80^oC. Multiple freeze/thaw cycles not recommended.