Wildtype phosphoinositide-dependent kinase-1 cDNA was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.   Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the kinase-1 cDNA expression cassette, generating a replication deficient recombinant virus. 

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