MFLM-91U cells, derived from mouse fetal lung mesenchyme.
Details:
Catalog Number AMFLM-9
Quantity 1 ml
Price $250.00
Organism/Strain Mouse, FVB/N Strain
Morphology Immature endothelial cell-like
Characteristics Cells express proteins associated with endothelial cell phenotype including CD34, PECAM-1, vonWillebrand factor, VEGFR1 (flt1), VEGFR2 (flk-1) and cell-surface recognition site for lectin GSL B4. By PCR analysis, MFLM-91U have mRNA transcripts for Tie-1, Tie-2, Ang-1 and Ang-2. The cells also express vimentin and low levels of smooth muscle alpha actin. MFLM-91U take up acetylated LDL and on Matrigel form extensive multi-cell, tubular networks.
Supplied As Frozen cells containing 5% DMSO, 95% fetal calf serum (v/v)
Storage
Use cryopreserved cells to establish cultures immediately upon receipt. If stored prior to culture, it is preferable to store in the vapor phase of liquid N2 . Note- storage prior to culture is likely to result in diminished recovery of viable cells.
Tumerogneic Tumerogenic in nu/nu mice
QC Testing Culture
Titer
1 ml, 1 x 106 cells
References:
Akeson, A.L., et al. Embryonic Vasculogenesis by Endothelial Precursor Cells Derived from Lung Mesenchyme. Developmental Dynamics , 217:11-23, 2000.
