Human cDNA for amino acids 282-1068 of NFATc3 (nuclear factor of activated T-cells) [NFAT4] with 5’ flag tag was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.   Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the human cDNA fragment with 5’ flag tag expression cassette, generating a replication deficient recombinant virus.