Seven Hills Bioreagents

Ad5-CMV-MEK1 activated

Download DatasheetDownload datasheet
Catalog NumberJMAd-37
Quantity0.5 ml
Category
  • MAP Kinase Related

GeneMEK1 activated
Vector ConstructionSer 217/221 Glu mutation in MAPK kinase 1 Rabbit cDNA was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the Rabbit cDNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingWestern Blot (to induce ERK1/2 phos)
TiterApprox 1E8/ml
References
Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng C-F, Kitsis RN, Molkentin JD. The MEK1 - ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice. EMBO J. 19:6341-6350. 2000 Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

Newsletter

Sign up to receive our newsletter, updates and our latest product releases.

We will not share, rent, or give your name or email address to anyone else. Please see our privacy policy if you have any questions.

Recently Published Articles

Chen G, Korfhagen TR, Karp CL, Impey S, Xu Y, Randell SH, Kitzmiller J, Maeda Y, Haitchi HM, Sridharan A, Senft AP, Whitsett JA.
Foxa3 induces goblet cell metaplasia and inhibits innate antiviral immunity. Am J Respir Crit Care Med. 2014 Feb 1;189(3):301-13.

Balli D, Ustiyan V, Zhang Y, Wang IC, Masino AJ, Ren X, Whitsett JA, Kalinichenko VV, Kalin TV.
Foxm1 transcription factor is required for lung fibrosis and epithelial-to-mesenchymal transition. EMBO J. 2013 Jan 23;32(2):231-44.

Dong M et al.
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Mol. Biol. Cell 2008;19:2620-2630.

Stahlman MT et al.
Expression of ABCA3 in developing lung and other tissues. J Histochem Cytochem 2007;55:71-83.