Vector ConstructionMouse cDNA for the adenine nucleotide translocase 1 (inner mitochondrial membrane transporter). It was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region with the mouse cDNA for Ant1, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
Storage Store at -80°C. Multiple freeze/thaw cycles not recommended.
QC TestingWestern Blot
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.
For research purposes only; not approved for diagnostic purposes or use in humans.