C4 reacts with all known actins from yeast to man with no known spurious reactivities. The C4 antibody is an excellent loading control for Western blots.
SpecificityAll known actins from yeast to man
ImmunogenChicken gizzard actin
- Cytoskeletal Antibodies
& suggested dilutionsApplications &suggested dilutions Immunoblots - 1:500 (DAB/peroxidase detection)Indirect immunofluorescence - 1:500Immunohistochemistry - 1:500, Blocking conditions: human specimens: 2% serum, mouse specimens: MOM kit (Vector Labs)
Supplied AsAscites fluid containing 0.01% Na azide.
StorageStorage at -70°C recommended, however the antibody is stable for months at -20°C and for weeks at 4°C. Multiple freeze/thaw cycles not recommended.