Seven Hills Bioreagents

Ad5-CMV-Cyclophilin D-flag

Download DatasheetDownload datasheet
Catalog NumberJMAd-87
Quantity0.5 ml
Category
  • Apoptosis / Mitochondria
  • Cyclophilin

GeneCyclophilin D-flag
Vector ConstructionMouse cyclophilin D cDNA with 5’flag tag was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cyclophilin D cDNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagFlag
QC TestingWestern Blot
TiterApprox 1E8/ml
References
Unpublished

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.

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