GeneERK2 dominant negative (AEF mutation) Vector ConstructionRat cDNA for dominant negative extracellular signal-regulated kinase (ERK2) cDNA, with, 5’ flag tag was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the rat cDNA, expression cassette, generating a replication deficient recombinant virus Supplied AsCrude HEK293 cell lysate. StorageStore at -80°C. Multiple freeze/thaw cycles not recommended. Epitope TagFlag QC TestingWestern Blot TiterApprox 1E8/ml
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.