GeneJNK2-mutant estrogen receptor fusion (tamoxifen-inducible)
Vector Construction Mouse C-Jun N-terminal kinase (JNK2) cDNA fused to the mutant estrogen receptor was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. This generates a tamoxifen inducible version of JNK2.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
QC TestingWestern Blot
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.