GeneM3/6 phosphatase (DUSP8)
Vector ConstructionMouse cDNA for the dual-specificity MAPK phosphatase M3/6, specific for JNK dephosphorylation, was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cDNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
QC TestingWestern Blot (to dephosphorylate JNK preferentially)
Liang Q, Bueno OF, Wilkins BJ, Jones F, Kimball TF, Kuan C-Y, Xia Y, Molkentin JD. C-Jun NH2-terminal kinases (JNK) antagonizes adaptive cardiac growth through cross-talk with the calcineurin-NFAT signaling pathway. EMBO J. 22:5059-5101 2003.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.