Vector ConstructionA dominant negative mutant of MEF2C (myocyte enhancer factor 2), amino acids 1-105 with R24L mutation, mouse cDNA was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. Homologous recombination between the two plasmids resulted in the replacement of the Ad5 early region 1 with the mouse cDNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
QC TestingWestern Blot
Xu J, Gong NL, Bodi I, Aronow BJ, Backx PH, Molkentin JD. MEF2A and MEF2C induce dilated cardiomyopathy in transgenic mice. J. Biol. Chem. 2006. In press.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992) 267:25129-25134.