Genep38-alpha-mutant estrogen receptor fusion (fusion protein activated by tamoxifen)
Vector ConstructionHuman p38alpha cDNA fused to mutant estrogen receptor was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome. This generates a tamoxifen inducible version of p38 beta.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
QC TestingWestern Blot
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.