Seven Hills Bioreagents


Download DatasheetDownload datasheet
Catalog NumberJMAd-33
Quantity0.5 ml
  • Transcription Factors

GeneMEF2-luciferase reporter
Vector ConstructionReporter adenovirus for MEF-2 DNA binding activity and transcriptional activation multimerized MEF2 sites upstream of a minimal TATA-containing promoter was fused to a luciferase cDNA cassette (the CMV promoter was removed). It was cloned into the multiple cloning site of pAC-CMV-pLpA from which the CMV promoter was removed. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 containing the Ad5 genome. Homologous recombination between the two plasmids resulted in replacement of Ad5 early region 1 with MEF-2 DNA expression cassette, generating a replication deficient recombinant virus.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Epitope TagNone
QC TestingNone
TiterApprox 1E8/ml
Wilkins BJ, Dai Y-S, Bueno OF, Parsons SA, Xu j, Plank DM, Jones F, Kimball TR, Molkentin JD. Calcineurin/NFAT coupling participates in pathologic, but not physiologic cardiac hypertrophy. Circ. Res. 94:110-118 2004.

Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.


Sign up to receive our newsletter, updates and our latest product releases.

We will not share, rent, or give your name or email address to anyone else. Please see our privacy policy if you have any questions.

Recently Published Articles

Chen G, Korfhagen TR, Karp CL, Impey S, Xu Y, Randell SH, Kitzmiller J, Maeda Y, Haitchi HM, Sridharan A, Senft AP, Whitsett JA.
Foxa3 induces goblet cell metaplasia and inhibits innate antiviral immunity. Am J Respir Crit Care Med. 2014 Feb 1;189(3):301-13.

Balli D, Ustiyan V, Zhang Y, Wang IC, Masino AJ, Ren X, Whitsett JA, Kalinichenko VV, Kalin TV.
Foxm1 transcription factor is required for lung fibrosis and epithelial-to-mesenchymal transition. EMBO J. 2013 Jan 23;32(2):231-44.

Dong M et al.
ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Mol. Biol. Cell 2008;19:2620-2630.

Stahlman MT et al.
Expression of ABCA3 in developing lung and other tissues. J Histochem Cytochem 2007;55:71-83.