Gene NFAT-luciferase reporter (reporter adenovirus for nuclear factor of activated T-cells activity and calcineurin activation)
Vector ConstructionNFAT- luciferase reporter that contains 9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase (lacking the CMV promoter) was cloned into the multiple cloning site of pAC-CMV-pLpA. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.
Supplied AsCrude HEK293 cell lysate.
StorageStore at -80°C. Multiple freeze/thaw cycles not recommended.
Wilkins BJ, Dai Y-S, Bueno OF, Parsons SA, Xu j, Plank DM, Jones F, Kimball TR, Molkentin JD. Calcineurin/NFAT coupling participates in pathologic, but not physiologic cardiac hypertrophy. Circ. Res. 94:110-118 2004.
Recombinant adenovirus was generated as described in Gomez-Foix, AM, et al., J. Biol. Chem. (1992)267:25129-25134.