B4 reacts selectively with enteric smooth muscle gamma-actin at high dilutions and does not react to either cytoplasmic ß- or gamma-actin.
SpecificityReacts selectively with enteric smooth muscle
ImmunogenChicken gizzard actin
- Cytoskeletal Antibodies
& suggested dilutionsTo be selective for smooth muscle actin: Immunoblots - 1:2000
Indirect immunofluorescence - 1:100-1:500
Immunohistochemistry - 1:500
Blocking conditions: human specimens: 2% serum
mouse specimens: MOM kit (Vector Labs)
Supplied AsAscites fluid containing 0.01% Na azide.
StorageStore at -70°C. Multiple freeze/thaw cycles not recommended.