The MFLM-4 cell line is an immortalized, isolated and cloned cell line, derived from mouse fetal lung mesenchyme. MFLM-4 cells express SV40 large T antigen and neomycin-resistance genes. The MFLM-4 cell line was isolated at E14.5 when the developing airways are branched epithelial tubules surrounded by a thick mesenchyme.
MorphologyImmature endothelial cell-like
CharacteristicsCells express proteins associated with endothelial cell phenotype including CD34, PECAM-1, vonWillebrand factor, VEGFR1 (flt1), VEGFR2 (flk-1) and cell-surface recognition site for lectin GSL B4. By PCR analysis, MFLM-4 have mRNA transcripts for Tie-1 (very low), Tie-2, Ang-1 and Ang-2. The cells also express vimentin and low levels of smooth muscle alpha actin. MFLM-4 take up acetylated LDL and on culture in Matrigel form tube-like structures with lumens.
Supplied AsFrozen cells containing 5% DMSO, 95% fetal calf serum (v/v).
StorageUse cryopreserved cells to establish cultures immediately upon receipt. If stored prior to culture, it is preferable to store in the vapor phase of liquid N2. Note- storage prior to culture is likely to result in diminished recovery of viable cells.
TumerogenicTumerogenic in nu/nu mice
Titer1 x 106 cells
Akeson, A.L., et al. Embryonic Vasculogenesis by Endothelial Precursor Cells Derived from Lung Mesenchyme. Developmental Dynamics, 217:11-23, 2000.
Akeson, A.L. In vitro Model for Developmental Progression form Vasculogenesis to Angiogenesis with a Murine Endothelial Precursor Cell Line, MFLM-4. Microvascular Research 61;75-86, 2001.