The MFLM-91U cell line is an immortalized, isolated and cloned cell line, derived from mouse fetal lung mesenchyme. MFLM-91U cells express SV40 large T antigen and neomycin-resistance genes. The cell line was isolated at E19 and adapted to grow in serum free medium.
MorphologyImmature endothelial cell-like
CharacteristicsCells express proteins associated with endothelial cell phenotype including CD34, PECAM-1, vonWillebrand factor, VEGFR1 (flt1), VEGFR2 (flk-1) and cell-surface recognition site for lectin GSL B4. By PCR analysis, MFLM-91U have mRNA transcripts for Tie-1, Tie-2, Ang-1 and Ang-2. The cells also express vimentin and low levels of smooth muscle alpha actin. MFLM-91U take up acetylated LDL and on Matrigel form extensive multi-cell, tubular networks.
Supplied AsFrozen cells containing 5% DMSO, 95% fetal calf serum (v/v)
StorageUse cryopreserved cells to establish cultures immediately upon receipt. If stored prior to culture, it is preferable to store in the vapor phase of liquid N2. Note- storage prior to culture is likely to result in diminished recovery of viable cells.
TumerogenicTumerogenic in nu/nu mice
Titer1ml, 1 x 106 cells
Akeson, A.L., et al. Embryonic Vasculogenesis by Endothelial Precursor Cells Derived from Lung Mesenchyme. Developmental Dynamics, 217:11-23, 2000.